捻转血矛线虫阿苯达唑耐药株给药前后比较转录组学分析

doi:10.11843/j.issn.0366-6964.2019.03.019

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (3): 637-644.doi: 10.11843/j.issn.0366-6964.2019.03.019

捻转血矛线虫阿苯达唑耐药株给药前后比较转录组学分析

赵学亮¹, 王姝懿^1,2, 呼和巴特尔¹, 孙柯¹, 苏倩¹, 吕旭¹, 王文龙^1*, 刘春霞^3*

1. 内蒙古农业大学兽医学院, 农业部动物疾病临床诊疗技术重点实验室, 呼和浩特 010018;
2. 内蒙古自治区综合疾病预防控制中心, 呼和浩特 010018;
3. 内蒙古农业大学生命科学院, 呼和浩特 010018

收稿日期:2018-09-10 出版日期:2019-03-23 发布日期:2019-03-23
通讯作者: 王文龙,主要从事寄生虫组学与分子免疫学研究,E-mail:wwl.imau@163.com;刘春霞,主要从事动物细胞发育分化调控机制研究,E-mail:lcx.imau@163.com
作者简介:赵学亮(1992-),男,河北沧州人,硕士生,主要从事寄生虫组学与分子免疫学研究,E-mail:zhaoxueliang92@sina.com
基金资助:
国家自然科学基金（31760731）；内蒙古自治区科技计划项目（201702074）

Comparative Transcriptome Analysis between before and after Administration of Albendazole Resistant Strain of Haemonchus contortus

ZHAO Xueliang¹, WANG Shuyi^1,2, HU Hebateer¹, SUN Ke¹, SU Qian¹, LÜ Xu¹, WANG Wenlong^1*, LIU Chunxia^3*

1. Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture, College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Inner Mongolia Center for Disease Control and Prevention, Hohhot 010018, China;
3. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2018-09-10 Online:2019-03-23 Published:2019-03-23

摘要/Abstract

摘要：

为探明捻转血矛线虫阿苯达唑耐药株给药前后在转录组水平上的差异，本研究采用Illumina Hiseq4000对给药前后的捻转血矛线虫进行转录组测序，筛选得到差异表达基因并通过GO和KEGG数据库对其进行功能注释及富集性分析，并利用荧光定量PCR验证部分差异表达基因。结果显示，共筛选获得851个显著差异表达基因，其中包括584个上调基因和267个下调基因。通过GO功能富集分析显示，有458、418和367个基因分别注释到生物学过程、分子功能和细胞组分三大类；对差异表达基因进行KEGG富集分析显示，有173个差异表达基因参与到75条KEGG通路中，显著富集在核糖体合成、细胞凋亡、过氧化物酶增殖体激活受体（PPAR）等信号通路。本试验初步筛选了阿苯达唑耐药株给药前后的差异表达基因，为深入探索捻转血矛线虫耐药性分子机制、筛选对耐药性检测的分子标记及耐药性早期鉴别诊断方法的建立提供重要数据。

Abstract:

Aiming to find the transcription difference of albendazole resistant strain of Haemonchus contortus,before and after administration. In this study, Samples of bBZ (before albendazole administration) and aBZ (after albendazole administration) were analyzed using Illumina Hiseq4000 RNA sequencing platform and their transcriptome libraries were constructed. Screening for differentially expressed genes(DEGs) and functional annotation and enrichment analysis by GO and KEGG databases,at the same time,they were verified by real-time PCR. The results showed that:a total of 851 DEGs between aBZ and bBZ, of which 584 and 267 genes were up-and down-regulated, respectively. The DEGs were then searched against the GO and KEGG database for enrichment analysis. GO analysis showed that 458,418 and 367 DEGs were annotated into biological process, cellular component and molecular function. KEGG analysis showed that 173 differentially expressed genes were assigned to 75 KEGG pathways, and clustered significantly in ribosome synthesis, apoptosis-multiple species, PPAR signaling pathway, and so on. This experiment initially screened DEGs between bBZ and aBZ of Haemonchus contortus. The study provides a foundation for exploring the molecular mechanism of resistance to Haemonchus contortus, screening for molecular markers of drug resistance detection and the establishment of early differential diagnosis methods for drug resistance.

中图分类号:

S855.9

赵学亮, 王姝懿, 呼和巴特尔, 孙柯, 苏倩, 吕旭, 王文龙, 刘春霞. 捻转血矛线虫阿苯达唑耐药株给药前后比较转录组学分析[J]. 畜牧兽医学报, 2019, 50(3): 637-644.

ZHAO Xueliang, WANG Shuyi, HU Hebateer, SUN Ke, SU Qian, Lü Xu, WANG Wenlong, LIU Chunxia. Comparative Transcriptome Analysis between before and after Administration of Albendazole Resistant Strain of Haemonchus contortus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 637-644.

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捻转血矛线虫阿苯达唑耐药株给药前后比较转录组学分析

Comparative Transcriptome Analysis between before and after Administration of Albendazole Resistant Strain of Haemonchus contortus

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